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Cell-cell contact affects membrane integrity after intracellular freezing.

Author(s) : ACKER J. P., MCGANN L. E.

Type of article: Article

Summary

The response of cells to freezing depends critically on the presence of an intact cell membrane. The cumulative incidence of cells with a loss of membrane integrity and the cumulative incidence of intracellular ice formation were determined as a function of temperature. Cells in suspension and individual attached cells showed no significant difference in the number of cells that formed intracellular ice and those that lost membrane integrity. For cells in a monolayer, with cell-cell contact, intracellular ice formation did not result in the immediate disruption of the plasma membrane in the majority of cells. This introduces the potential for minimizing damage due to intracellular ice formation and for developing strategies for the cryoprotection of tissues during rapid cooling.

Details

  • Original title: Cell-cell contact affects membrane integrity after intracellular freezing.
  • Record ID : 2001-1093
  • Languages: English
  • Source: Cryobiology - vol. 40 - n. 1
  • Publication date: 2000/02

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