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Characterization of a psychrotrophic Arthrobacter gene and its cold-active beta-galactosidase.

Author(s) : TRIMBUR D. E., GUTSHALL K. R., PREMA P., BRENCHLEY J. E.

Type of article: Article

Summary

The authors isolated and characterized several psychrotrophic microorganisms. One isolate, B7, is an Arthrobacter strain which produces beta-galactosidase when grown in lactose minimal media. When chromosomal DNA was prepared and transformed into Escherichia coli, three different genes encoding beta-galactosidase activity were obtained. They subcloned and sequenced one of these beta-galactosidase genes from the Arthrobacter isolate B7. On the basis of amino acid sequence alignment, the gene was found to have probable catalytic sites homologous to those from the E. coli lacZ gene. The enzyme was purified and characterized.

Details

  • Original title: Characterization of a psychrotrophic Arthrobacter gene and its cold-active beta-galactosidase.
  • Record ID : 1995-3538
  • Languages: English
  • Source: Appl. environ. Microbiol. - vol. 60 - n. 12
  • Publication date: 1994/12
  • Document available for consultation in the library of the IIR headquarters only.

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