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Cold acclimation improves recovery of cryopreserved grass (Zoysia and Lolium sp.).

Author(s) : CHANG Y., BARKER R. E., REED B. M.

Type of article: Article

Summary

Cold acclimation of Lolium L. and Zoysia Willd. grass cultivars significantly increased regrowth of cryopreserved meristems. One week of cold acclimation improved recovery following cryopreservation but extended cold acclimation (4-8 weeks) resulted in the best regrowth. Cold acclimation also significantly increased the dehydration tolerance of both Zoysia and Lolium meristems. Lolium apices cold acclimated for 4 weeks produced 60-100% regrowth following cryopreservation by slow freezing or encapsulation-dehydration. Cold-acclimated Zoysia had greater than 60% regrowth following encapsulation-dehydration when beads were dehydrated to less than 22% water content.

Details

  • Original title: Cold acclimation improves recovery of cryopreserved grass (Zoysia and Lolium sp.).
  • Record ID : 2001-1405
  • Languages: English
  • Source: CryoLetters - vol. 21 - n. 2
  • Publication date: 2000/03
  • Document available for consultation in the library of the IIR headquarters only.

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