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CRYOINJURY OF ISOLATED PROTOPLASTS: A CONSEQUENCE OF DEHYDRATION OR THE FRACTION OF THE SUSPENDING MEDIUM THAT IS FROZEN.

Author(s) : STEPONKUS P. L., GORDON-KAMM W. J.

Type of article: Article

Summary

PROTOPLASTS ISOLATED FROM NON-ACCLIMATED LEAVES OF RYE (SECALE CEREALE L CV PUMA) WERE SUSPENDED IN VARIED CONCENTRATIONS OF SORBITOL SO AS TO EFFECT THE MAXIMUM POSSIBLE RANGE OF L (FRACTION OF UNFROZEN EXTRACELLULAR SOLUTION) AND U (FRACTION OF UNFROZEN EXTRACELLULAR WATER) VALUES AT FIXED BELOW 273 K (0 DEG C) TEMPERATURES OVER THE RANGE OF 273 TO 258 K (0 TO -15 DEG C). SURVIVAL DECLINED FROM 100 TO LOWER THAN 10% OVER THIS TEMPERATURE RANGE AND WAS INDEPENDENT OF THE L OR U VALUE. SURVIVAL WAS, HOWEVER, A FUNCTION OF CELL DEHYDRATION WHEN EXPRESSED AS THE FRACTIONAL OSMOTIC VOLUME OF THE PROTOPLASTS.

Details

  • Original title: CRYOINJURY OF ISOLATED PROTOPLASTS: A CONSEQUENCE OF DEHYDRATION OR THE FRACTION OF THE SUSPENDING MEDIUM THAT IS FROZEN.
  • Record ID : 1986-0883
  • Languages: English
  • Source: Cryo-Letters - vol. 6 - n. 4
  • Publication date: 1985
  • Document available for consultation in the library of the IIR headquarters only.

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