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THE RESPONSES OF 4 CULTURED PLANT CELL LINES TO FREEZING AND THAWING IN THE PRESENCE OR ABSENCE OF CRYOPROTECTANT MIXTURES.

Author(s) : MCLELLAN M. R., SCHRIJNEMAKERS E. W. M., IREN F. van

Type of article: Article

Summary

TWO PLANT CELL SUSPENSION LINES (CATHARANTHUS ROSEUS AND TABAERNAEMONTANA DIVARICATA LINE 21) WERE RECOVERED FOLLOWING FREEZING TO 77 K (-196 DEG C) AT ACCEPTABLE RATE TWO OTHER CELL LINES (CINCHONA ROBUSTA AND TABAERNAEMONTANA DIVARICATA LINE 6) WERE RECALCITRANT TO THESE SAME PROCEDURES. A CRYOMICROSCOPE STUDY INDICATED THAT THE RECALCITRANT LINES SUFFERED A RANGE OF LESIONS DURING FREEZING AND THAWING. A SIGNIFICANT PROPORTION OF THE CELLS OF RESISTANT LINES DEMONSTRATED NONE OF THESE LESIONS WHEN FROZEN IN SUCROSE/DMSO/GLYCEROL (SDG) CRYOPROTECTANT MIXTURES.

Details

  • Original title: THE RESPONSES OF 4 CULTURED PLANT CELL LINES TO FREEZING AND THAWING IN THE PRESENCE OR ABSENCE OF CRYOPROTECTANT MIXTURES.
  • Record ID : 1991-0874
  • Languages: English
  • Source: Cryo-Letters - vol. 11 - n. 3
  • Publication date: 1990/05
  • Document available for consultation in the library of the IIR headquarters only.

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