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CRYOPRESERVATION AND FREEZE-DRYING OF MICRO-ORGANISMS.

Author(s) : SIDYAKINA T. M.

Summary

FREEZE-DRYING HAS BEEN SUCCESSFULLY USED FOR A LONG TIME IN THE STORAGE OF MICRO-ORGANISMS. MANY NONSPORULATING MICROBIAL CULTURES, THOUGH, POSSESS LITTLE TOLERANCE FOR FREEZE-DRYING PROCEDURES AND HAVE A LOW VIABILITY WHEN PRESERVED BY THIS TECHNIQUE, THEIR VIABILITY DECLINING DURING STORAGE EVEN AT A TEMPERATURE OF 277 K (4 DEG C). CRYOPRESERVATION OR STORAGE AT LIQUID NITROGEN TEMPERATURE: 77 K (-196 DEG C), BEGAN TO BE USED FOR PRESERVING MICRO-ORGANISMS RATHER RECENTLY. WITH CRYOPRESERVATION, THE RELATIVE NUMBER OF VIABLE CELLS IS SUB-STANTIALLY HIGHER THAN WITH FREEZE-DRYING, AND THE VIABILITY IS RETAINED DURING LONG-TERM STORAGE IN LIQUID NITROGEN. FOR THIS REASON, THE POSSIBLE PERIOD OF TIME FOR WHICH MICRO-ORGANISMS CAN BE STORED BECOMES UNLIMITED. NEVERTHELESS, TO CRYOPRESERVE MOST NONSPORULATING MICRO-ORGANISMS (ESPECIALLY EUCARYOTES, FUNGI AND YEASTS) SUCCESSFULLY, PRELIMINARY PROGRAMMED FREEZING, WITH SELECTION OF THE MOST EFFECTIVE ING RATES, IS NECESSARY. THE AIM OF THE AUTHOR'S INVESTIGATIONS WAS TO STUDY THE EFFECTS OF FREEZE-DRYING AND CRYOPRESERVATION ON VIABILITY, BIOCHEMICAL PROPERTIES AND GENETIC STABILITY OF MICRO-ORGANISMS.

Details

  • Original title: CRYOPRESERVATION AND FREEZE-DRYING OF MICRO-ORGANISMS.
  • Record ID : 1990-2131
  • Languages: English
  • Publication date: 1989/07/06
  • Source: Source: Proc. IVth int. Sch. Cryobiol. Freeze-drying, Borovets
    L155-L157; 1 fig.; 4 tabl.; 12 ref.
  • Document available for consultation in the library of the IIR headquarters only.

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