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CRYOPRESERVATION AND TRANSPLANTATION OF MORULA EMBRYOS IN MOUSE.

Author(s) : ZHOU J.

Summary

EMBRYOS AT THE MORULA STAGE WERE COLLECTED FROM SUPEROVULATED AND NONSUPEROVULATED MICE. THEY WERE FROZEN IN PLASTIC VIALS CONTAINING A PHOSPHATE-BUFFERED SALINE (PBS) MEDIA WITH 1.5 M GLYCEROL WHICH WAS COOLED GRADUALLY FROM ROOM TEMPERATURE TO 266 K (-7 DEG C). SEEDING CRYSTAL WAS MAINTAINED AT THIS EQUILIBRIUM TEMPERATURE FOR 10 MINUTES. THEN, THEY WERE FROZEN TO 238/223 K (-35/-50 DEG C), AND FINALLY PLUNGED INTO LIQUID NITROGEN. WHEN THE EMBRYOS WERE TO BE TRANSPLANTED, THE FROZEN MASS WAS THAWED AND GLYCEROL WAS REMOVED WITH 1 M SUCROSE. THE EMBRYOS WERE THEN TRANSFERRED INTO PBS. FIFTYNINE PERCENT OF THE 1,892 CRYOPRESERVED EMBRYOS WERE CONSIDERED AS < SURVIVED >. THE LIVE-BIRTH RATE REACHED 23.44%.

Details

  • Original title: CRYOPRESERVATION AND TRANSPLANTATION OF MORULA EMBRYOS IN MOUSE.
  • Record ID : 1991-0888
  • Languages: English
  • Publication date: 1989/10/11
  • Source: Source: Proc. 7th int. Congr. Cryosurg., Beijing
    239-242; 2 fig.; 2 tabl.; 4 ref.
  • Document available for consultation in the library of the IIR headquarters only.

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