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Cryopreservation of apple shoot tips: importance of cryopreservation technique and of conditioning of donor plants.

Author(s) : WU Y., ENGELMANN F., ZHOU M., CHEN S., ZHAO Y.

Type of article: Article

Summary

In the paper, the efficiency of three techniques (two-step freezing, vitrification, encapsulation-dehydration) for freezing in vitro shoot tips was compared and the effect of conditioning of mother-plants was investigated. The best results (up to 86% regrowth after cryopreservation without any callusing) where obtained with the encapsulation-dehydration technique. Simplified encapsulation-dehydration protocol was successfully applied to 11 out of the 12 cultivars tested.

Details

Original title: Cryopreservation of apple shoot tips: importance of cryopreservation technique and of conditioning of donor plants.
Record ID : 2000-0237
Languages: English
Source: Cryo-Letters - vol. 20 - n. 2
Publication date: 1999/03
Document available for consultation in the library of the IIR headquarters only.

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Indexing

Themes: Food engineering;
Seeds and plants
Keywords: Tree (plant); Recommendation; Process; Apple; Shoot; Cryopreservation