Summary
CHONDROCYTES ISOLATED FROM HUMAN FETAL ARTICULAR CARTILAGE WERE CRYOPRESERVED. THE OPTIMAL COOLING RATE FOR CHONDROCYTES WAS SELECTED BY PROGRAM CONTROLLED FREEZING AND THE BEST SURVIVAL RATE (94.25%) WAS OBTAINED WHEN THE COOLING RATE WAS 4 K/MIN. DIFFERENT THAWING CURVES WERE OBSERVED. THAWING IN A 310-313 K (37-40 DEG C) WATER BATH GOT THE BEST RESULT. THERE WAS NO OBVIOUS CHANGE OF VIABILITIES OF CHONDROCYTES DURING 120 DAYS STORAGE AT 77 K (-196 DEG C).
Details
- Original title: CRYOPRESERVATION OF ARTICULAR CARTILAGE CHONDROCYTES.
- Record ID : 1991-0884
- Languages: English
- Publication date: 1989/10/11
- Source: Source: Proc. 7th int. Congr. Cryosurg., Beijing
224-228; 5 fig.; 8 ref. - Document available for consultation in the library of the IIR headquarters only.
Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Cartilage; Tissue; Cryopreservation
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BIOLOGICAL EFFECTS OF FREEZING ON VARIOUS TISSUES.
- Author(s) : DING H. C.
- Date : 1989/07/06
- Languages : English
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From the tissue bank to the tissue establishment.
- Author(s) : MERICKA P., STRAKOVĂ H., HORYNOVĂ A.
- Date : 2008/04/21
- Languages : English
- Source: Cryogenics 2008. Proceedings of the 10th IIR International Conference
- Formats : PDF
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LOW TEMPERATURE PRESERVATION OF COLLAGENIC TISS...
- Author(s) : MERICKA P.
- Date : 1987/08/24
- Languages : English
- Source: Development in refrigeration, refrigeration for development. Proceedings of the XVIIth international Congress of Refrigeration.
- Formats : PDF
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PRACTICAL METHODS FOR PRESERVATION OF CULTURED ...
- Author(s) : OHNISHI K.
- Date : 1985/05/20
- Languages : English
- Source: Fundamentals and Applications of Freeze-Drying to Biological Materials, Drugs and Foodstuffs.
- Formats : PDF
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TISSUE PRESERVATION.
- Author(s) : ARMITAGE W. J.
- Date : 1989/07/06
- Languages : English
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