Summary
CHONDROCYTES ISOLATED FROM HUMAN FETAL ARTICULAR CARTILAGE WERE CRYOPRESERVED. THE OPTIMAL COOLING RATE FOR CHONDROCYTES WAS SELECTED BY PROGRAM CONTROLLED FREEZING AND THE BEST SURVIVAL RATE (94.25%) WAS OBTAINED WHEN THE COOLING RATE WAS 4 K/MIN. DIFFERENT THAWING CURVES WERE OBSERVED. THAWING IN A 310-313 K (37-40 DEG C) WATER BATH GOT THE BEST RESULT. THERE WAS NO OBVIOUS CHANGE OF VIABILITIES OF CHONDROCYTES DURING 120 DAYS STORAGE AT 77 K (-196 DEG C).
Details
- Original title: CRYOPRESERVATION OF ARTICULAR CARTILAGE CHONDROCYTES.
- Record ID : 1991-0884
- Languages: English
- Publication date: 1989/10/11
- Source: Source: Proc. 7th int. Congr. Cryosurg., Beijing
224-228; 5 fig.; 8 ref. - Document available for consultation in the library of the IIR headquarters only.
Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Cartilage; Tissue; Cryopreservation
-
Effect of cryopreservation on human articular c...
- Author(s) : RENDAL-VÁZQUEZ M. E., MANEIRO-PAMPÍN E., RODRÍGUEZ-CABARCOS M., et al.
- Date : 2001/02
- Languages : English
- Source: Cryobiology - vol. 42 - n. 1
View record
-
The value of frozen cartilage tissues without c...
- Author(s) : CETINKAYA G., HATIPOGLU I., ARAT S.
- Date : 2014/02
- Languages : English
- Source: Cryobiology - vol. 68 - n. 1
View record
-
A simple cryopreservation method for the mainte...
- Author(s) : OEGEMA T. R. Jr, DELORIA L. B., FEDEWA M. M., et al.
- Date : 2000/06
- Languages : English
- Source: Cryobiology - vol. 40 - n. 4
View record
-
Cryopreservation of porcine articular cartilage...
- Author(s) : LAOUAR L., FISHBEIN K., MCGANN L. E., et al.
- Date : 2007/02
- Languages : English
- Source: Cryobiology - vol. 54 - n. 1
View record
-
A refinement to the liquidus-tracking method fo...
- Author(s) : YU W., CHENG G., ZHANG S.
- Date : 2013/05
- Languages : English
- Source: CryoLetters - vol. 34 - n. 3
View record