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Cryopreservation of coffee (Coffea arabica L.) seeds: importance of the precooling temperature.

Author(s) : DUSSERT S., CHABRILLANGE N., ENGELMANN F., ANTHONY F., HAMON S.

Type of article: Article

Summary

The effect of the precooling temperature on the survival rate of cryopreserved Coffea arabica seeds and excised zygotic embryos was investigated. The optimal germination rate (70%) of cryopreserved seeds was achieved after precooling to -50 deg C. After 4 months in culture, 30% of seeds developed into normal seedlings. For embryos extracted after thawing from cryopreserved seeds, maximal survival rate (97%) was observed when seeds were immersed into liquid nitrogen directly after dehydration, without precooling. All viable embryos developed into normal seedlings after 2 months in culture.

Details

  • Original title: Cryopreservation of coffee (Coffea arabica L.) seeds: importance of the precooling temperature.
  • Record ID : 1998-1967
  • Languages: English
  • Source: Cryo-Letters - vol. 18 - n. 5
  • Publication date: 1997/09
  • Document available for consultation in the library of the IIR headquarters only.

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