ShareFreeze-preservation of Catharanthus protoplasts at liquid nitrogen temperature: comparison with cells.
Author(s) : GAZEAU C. M., BLANCHON C., DEREUDDRE J.
Type of article: Article
Summary
The viability of cryoprotected protoplasts was evaluated by different staining methods and estimated immediately after thawing and one day later: survival percentages were 50 and 20% respectively. Survival rates of cryoprotected cells subjected to the same conditions were slightly higher (60 and 35% respectively). Two days after rewarming, cell wall regeneration began as patches on the protoplast surface.
Details
- Original title: Freeze-preservation of Catharanthus protoplasts at liquid nitrogen temperature: comparison with cells.
- Record ID : 1993-0537
- Languages: English
- Source: Cryo-Letters - vol. 13 - n. 3
- Publication date: 1992/05
- Document available for consultation in the library of the IIR headquarters only.
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- Date : 1992/05
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- Date : 1997/05
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- Source: Cryo-Letters - vol. 18 - n. 3
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- Date : 1998/05
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