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CRYOPRESERVATION OF FETAL RAT BRAIN TISSUE LATER USED FOR INTRACEREBRAL TRANSPLANTATION.

Author(s) : JENSEN S., SORENSEN T., ZIMMER J.

Type of article: Article

Summary

A METHOD FOR CRYOPRESERVATION OF IMMATURE RAT CENTRAL NERVOUS TISSUE IS PRESENTED. THE METHOD WAS APPLIED TO BRAIN TISSUE FROM 16-AND 17-DAY-OLD FETAL RATS, INCLUDING THE NEOCORTEX, HABENULA, SEPTUM AND BASAL FOREBRAIN, CEREBELLUM, AND RETINA. AFTER STORAGE IN LIQUID NITROGEN FROM 6 TO 52 DAYS THE TISSUE WAS GRAFTED INTO THE BRAIN OF ADULT RATS. THE RECIPIENTS SURVIVED FOR 23 TO 673 DAYS BEFORE THEIR BRAINS WERE PROCESSED BY CURRENT NEUROANATOMICAL, HISTOLOGICAL METHODS. THE AUTHORS CONCLUDE THAT CRYOPRESERVATION IS A RELIABLE METHOD FOR STORAGE OF IMMATURE NEURAL TISSUE LATER TO BE USED FOR INTRACEREBRAL GRAFTING.

Details

  • Original title: CRYOPRESERVATION OF FETAL RAT BRAIN TISSUE LATER USED FOR INTRACEREBRAL TRANSPLANTATION.
  • Record ID : 1987-2448
  • Languages: English
  • Source: Cryobiology - vol. 24 - n. 2
  • Publication date: 1987

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