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Cryopreservation of embryonic cerebral tissue of rat.

Author(s) : FANG J., ZHANG Z. X.

Type of article: Article

Summary

Embryonic cerebral tissues (ECT) either fresh or frozen-stored, were cultured and transplanted into the cerebella of neonatal host rats. Many variables including composition of the freezing medium, freezing and thawing rates, and storage time in liquid nitrogen were studied systematically. The results indicated that the following conditions yielded good results for tissue culture: using 1 M dimethyl sulfoxide as the cryoprotectant, freezing the brain tissues at a rate of 1 K/min until it reached -70 deg C, storing the frozen samples in liquid nitrogen and thawing them fast in a 37 deg C water bath. The viability of the frozen-thawed tissues was assessed by their abilities to grow and differentiate in vitro and in vivo after intracerebral grafting. Detailed results are given. From this and similar studies on the subject by others, the authors conclude that cryopreservation is a feasible method for storage of embryonic brain tissue to be used later for intracerebral grafting.

Details

  • Original title: Cryopreservation of embryonic cerebral tissue of rat.
  • Record ID : 1993-0530
  • Languages: English
  • Source: Cryobiology - vol. 29 - n. 2
  • Publication date: 1992/04

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