Share

Cryopreservation of human failed maturation oocytes shows that vitrification gives superior outcomes to slow cooling.

Author(s) : FASANO G., VANNIN A. S., BIRAMANE J., et al.

Type of article: Article

Summary

This study investigated whether failed maturation oocytes could be used to evaluate different cryopreservation procedures. A total of 289 failed maturation oocytes (GV and MI stages), obtained from 169 patients undergoing IVF treatment (mean age 33.84 ± 5.0) were divided into two different slow-cooling groups (1.5 mol/l 1,2-propanediol + 0.2 mol/l sucrose in either NaCl (group A) or choline chloride (ChCl) (group B) based cryopreservation solutions) and one vitrification group (15% ethylene glycol + 15% dimethyl sulphoxide). Survival rate, in vitro maturation (IVM) rate, fertilization and developmental rate of cryopreserved oocytes were assessed. Regardless of the stage at which cryopreservation was performed (GV + MI), the slow cooling with ChCl based medium always gave significantly lower survival rate than the slow cooling in NaCl based medium (p = 0.01) and vitrification (p < 0.001). An extended study also showed statistically reduced survival rate between slow-cooling NaCl based medium and vitrification (p < 0.05). Global results of in vitro maturation and fertilization showed worse results between both slow-cooling NaCl and ChCl based media versus vitrification. In conclusion, for oocytes that had failed to mature, vitrification gave better survival, maturation, fertilization and also cleavage rates than the slow-cooling protocols. Four cells embryos were obtained only from vitrified in vitro matured MI oocytes. [Reprinted with permission from Elsevier. Copyright, 2010].

Details

  • Original title: Cryopreservation of human failed maturation oocytes shows that vitrification gives superior outcomes to slow cooling.
  • Record ID : 30003651
  • Languages: English
  • Source: Cryobiology - vol. 61 - n. 3
  • Publication date: 2010/12
  • DOI: http://dx.doi.org/10.1016/j.cryobiol.2010.08.002

Links


See other articles in this issue (4)
See the source

Indexing