Share

Cryopreservation of human sperm using rapid cooling rates.

Author(s) : GRISCHENKO V. I., DUNAEVSKAYA A. V., BABENKO V. I.

Type of article: Article

Summary

The majority of protocols used for cryopreservation of human spermatazoa involve low cooling rates. However, calculations have shown that an optimal cooling rate is about 7 x 10 000 °C/min. The authors studied the effect of high cooling rates, variation of osmolarity of the cryoprotectant medium and the glycerol content. The results demonstrated that decreasing the cooling rate down to 1.75-2.5 x10 000 °C/min induced a statistically significant reduction in sperm motility. The cooling rate of 8-11 x 10 000 °C /min enabled only a small percentage of spermatozoa to retain their motility.

Details

  • Original title: Cryopreservation of human sperm using rapid cooling rates.
  • Record ID : 2004-0022
  • Languages: English
  • Source: CryoLetters - vol. 24 - n. 2
  • Publication date: 2003/03
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (4)
See the source

Indexing