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Cryopreservation of ovules and somatic embryos of citrus using the encapsulation-dehydration technique.

Author(s) : GONZÁLEZ ARNAO M. T., JUÁREZ J., ORTEGA C., et al.

Type of article: Article

Summary

Cryopreservation of ovules and somatic embryos from several genotypes of citrus was achieved using encapsulation-dehydration. Survival of cryopreserved ovules was occasional and erratic after different pregrowth conditions in liquid medium with 0.75 M, 1 M or up to 1.25 M sucrose. An efficient cryopreservation protocol was established for somatic embryos derived from two sources. High survival rates (75-100%) were consistently obtained after 1 day pregrowth in 0.75 M sucrose, desiccation down to 20-25% moisture content in the beads and direct immersion in liquid nitrogen. Embryos subjected to encapsulation-dehydration accumulated high sucrose levels which appear to ensure the recovery of the whole embryo after cryopreservation.

Details

  • Original title: Cryopreservation of ovules and somatic embryos of citrus using the encapsulation-dehydration technique.
  • Record ID : 2004-0019
  • Languages: English
  • Source: CryoLetters - vol. 24 - n. 2
  • Publication date: 2003/03
  • Document available for consultation in the library of the IIR headquarters only.

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