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Cryopreservation of mature embryos of coconut (cocos nucifera L.) and subsequent regeneration of plantlets.

Author(s) : ASSY-BAH B., ENGELMANN F.

Type of article: Article

Summary

Mature embryos (10 to 12 months after pollination) of four varieties of coconut (hybrid PB 121, Cameroon Red Dwarf, Indian Tall, Renell Tall) could withstand cryopreservation in liquid nitrogen and develop into plants. Pretreatment consisted of a 4-hour desiccation in the air current of a laminar flow cabinet followed by a 11 to 20-hour culture on a medium containing 600 g/l glucose and 15% glycerol. Freezing and thawing were carried out rapidly. Recovery rates varied between 33 and 93% of frozen embryos, depending on the variety.

Details

  • Original title: Cryopreservation of mature embryos of coconut (cocos nucifera L.) and subsequent regeneration of plantlets.
  • Record ID : 1993-0272
  • Languages: English
  • Source: Cryo-Letters - vol. 13 - n. 2
  • Publication date: 1992/03
  • Document available for consultation in the library of the IIR headquarters only.

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