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Cryopreservation of embryonic axes of an homeohydrous (recalcitrant) seed in relation to calorimetric properties of tissue water.

Author(s) : VERTUCCI C. W., et al.

Type of article: Article

Summary

A survival rate of 90% following freezing to -70 deg C and storage at that temperature for 6 months, was achieved for embryonic axes excised from desiccation-sensitive seeds of Landolphia kirkii. These had been very rapidly dehydrated to the optimal moisture level prior to freezing. Differential scanning calorimetry showed that the water in most of the surviving axes melted as a broad endothermic peak with onset temperature between -12 and -25 deg C. However, some survival (43%) was observed in axes in which no endotherm was oberved. There was no survival in axes where a portion of water melted from a pure form at 0 deg C.

Details

  • Original title: Cryopreservation of embryonic axes of an homeohydrous (recalcitrant) seed in relation to calorimetric properties of tissue water.
  • Record ID : 1993-0271
  • Languages: English
  • Source: Cryo-Letters - vol. 12 - n. 6
  • Publication date: 1991/11
  • Document available for consultation in the library of the IIR headquarters only.

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