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Cryopreservation of Citrus aurantifolia seeds and embryonic axes using a desiccation protocol.

Author(s) : CHO E. G., NOOR N. M., KIM H. H., et al.

Type of article: Article

Summary

The desiccation and freezing tolerance of seeds, with and without testas, and embryonic axes of Citrus aurantifolia were investigated. Seeds were desiccated with silica gel. Whatever the desiccation method employed, survival before and after cryopreservation was higher for seeds without testas. For intact seeds, the highest survival percentage (41.3%) was achieved after desiccation of 7.3% moisture content. Survival of seeds cryopreserved without testas reached up to 85% after desiccation, corresponding to moisture contents of 7.1 and 4.5%, respectively. After desiccation with silica gel, survival reached a maximum of 60% for a seed moisture content of 3.3%. Survival of control embryonic axes was high (80-100%) whatever the sucrose concentration in the preculture medium and the duration of the desiccation period. After cryopreservation, no survival was noted with embryonic axes, which had not been precultured nor desiccated. Survival of non-desiccated embryonic axes increased progressively with increasing sucrose concentrations in the preculture medium, from 7.5% with 0.1 M sucrose to 77.5% with 0.7 M sucrose. Survival of desiccated and cryopreserved embryos was always high, whatever the preculture treatment and desiccation period, ranging from 55.8 to 92.5%.

Details

  • Original title: Cryopreservation of Citrus aurantifolia seeds and embryonic axes using a desiccation protocol.
  • Record ID : 2003-3029
  • Languages: English
  • Source: CryoLetters - vol. 23 - n. 5
  • Publication date: 2002/09
  • Document available for consultation in the library of the IIR headquarters only.

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