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Cryopreservation of rainbow trout (Oncorhynchus mykiss) blastomeres.

Author(s) : NILSSON E., CLOUD J. G.

Type of article: Article

Summary

The objective of the investigation was to determine if the rate of cooling or the type of container alters cryopreservation success. Isolated blastomeres in a freezing solution containing 8.7% DMSO were subjected to one of two different cooling rates in either 0.5 ml French semen straws or cryovials. Thawed samples were examined using phase contrast microscopy, with cells scored as being either intact or non-intact. There was no significant difference between the cooling rates used in the paper based on the proportion of intact cells, but there was a significant difference in the proportion of intact cells between samples frozen in French straws (35.9 more or less 16.5%) and those frozen in cryovials (19.4 more or less 4.9%).

Details

  • Original title: Cryopreservation of rainbow trout (Oncorhynchus mykiss) blastomeres.
  • Record ID : 1994-0582
  • Languages: English
  • Source: Aquat. living Resour./Ressour. vivantes aquat. - vol. 6 - n. 1
  • Publication date: 1993
  • Document available for consultation in the library of the IIR headquarters only.

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