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A STUDY ON THE CRYOPRESERVATION OF COMMON CARP CYPRINUS CARPIO EMBRYOS.

Author(s) : ZHANG X. S.

Type of article: Article

Summary

A FEW EMBRYOS OF THE COMMON CARP CYPRINUS CARPIO COOLED TO LIQUID NITROGEN COULD SURVIVE AFTER THAWING. CRYOPRESERVATION OF FISH EMBRYOS IS STILL AN UNSOLVED PROBLEM WHICH DEPENDS NOT ONLY ON FREEZING RATES, WARMING RATES AND CONCENTRATIONS OF CRYOPROTECTIVE AGENTS, BUT ALSO ON EMBRYONIC QUALITY, EMBRYONIC STAGES AND SOME UNKNOWN EFFECTS. THE TAILBUD STAGE MIGHT BE THE APPROPRIATE STAGE FOR THE CRYOPRESERVATION. SOME SUCCESSFUL EXAMPLES: THE COOLING RATE WAS LESS THAN 0.07 K/MIN FROM THE FREEZING POINT TO 213 K (-60 DEG C) ; THE SLOW WARMING ABOUT 8 K/MIN, AND DMSO AS CRYOPROTECTIVE AGENT WAS SUPERIOR TO GLYCEROL.

Details

  • Original title: A STUDY ON THE CRYOPRESERVATION OF COMMON CARP CYPRINUS CARPIO EMBRYOS.
  • Record ID : 1990-2154
  • Languages: English
  • Source: Cryo-Letters - vol. 10 - n. 5
  • Publication date: 1989/09
  • Document available for consultation in the library of the IIR headquarters only.

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