ShareCRYOPRESERVATION OF SKIN USING A MURINE MODEL: VALIDATION OF A PROGNOSTIC VIABILITY ASSAY.
Author(s) : KEARNEY J. N., WHELDON L. A., GOWLAND G.
Type of article: Article
Summary
IN ORDER TO EVALUATE THE MANY VARIABLES THAT CAN AFFECT CRYOPRESERVATION SUCCESS, A SIMPLE, HIGHLY REPRODUCIBLE MODEL SYSTEM IS REQUIRED. THE AUTHORS HAVE EVALUATED THE USE OF TETRAZOLIUM REDUCTASE ACTIVITY AS A PROGNOSTIC INDICATOR OF SKIN VIABILITY IN AN INBRED MURINE MODEL. SKIN TETRAZOLIUM REDUCTASE ACTIVITY CORRELATED WELL WITH OXYGEN CONSUMPTION, AND WITH GRAFT SUCCESS FOLLOWING VARYING DEGREES OF CRYOGENIC INJURY. THE ASSAY WAS SHOWN TO BE HIGHLY REPRODUCIBLE.
Details
- Original title: CRYOPRESERVATION OF SKIN USING A MURINE MODEL: VALIDATION OF A PROGNOSTIC VIABILITY ASSAY.
- Record ID : 1991-0421
- Languages: English
- Source: Cryobiology - vol. 27 - n. 1
- Publication date: 1990
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Tissue; Survival; Mouse; Skin; Graft; Enzyme; Cryopreservation
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- Date : 1989/10/11
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