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CRYOPRESERVATION OF SKIN USING AN INSULATED HEAT SINK BOX STORED AT 203 K (-70 DEG C).

Author(s) : MAY S. R., GUTTMAN R. M., WAINWRIGHT J. F.

Type of article: Article

Summary

THE COOLING OF SKIN TO 203 K WAS CARRIED OUT BY TWO METHODS: PROGRAMMED CONTROLLED-RATE (PCR) COOLING AT 1 K/MIN TO 203 K, AND VARIABLE-RATE COOLING TO 203 K IN AN INSULATED HEAT SINK BOX (IHSB). THE IHSB CONTAINING SKIN WAS COOLED IN A 203 K CONSTANT--TEMPERATURE REFRIGERATOR ; THE INSULATION PROVIDES A SLOW COOLING RATE WHILE THE PAIRED HEAT SINKS PROVIDE EVEN HEAT FLOW ACROSS THE TOP AND BOTTOM SURFACES OF THE FLAT SKIN PACKETS, MINIMIZING THE DURATION AND POTENTIAL DAMAGING EFFECTS OF THE EXOTHERMIC TEMPERATURE PLATEAU WHICH OCCURS AT THE FREEZING POINT. WHEN FOLLOWED BY 24-HR STORAGE AT 203 K AND WARMING AT ABOUT 316 K/MIN, THE IHSB COOLING METHOD WAS EQUIVALENT TO THE PCR METHOD IN GENERATING A SUITABLY SLOW COOLING RATE OF 1 TO 2 K/MIN, AND MAINTAINING ABOUT 80% OF NORMAL SKIN CELL GLUCOSE METABOLISM. G.R.S.

Details

  • Original title: CRYOPRESERVATION OF SKIN USING AN INSULATED HEAT SINK BOX STORED AT 203 K (-70 DEG C).
  • Record ID : 1986-0887
  • Languages: English
  • Source: Cryobiology - vol. 22 - n. 3
  • Publication date: 1985

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