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Survival rate of frozen-thawed bovine IVF embryos in relation to exposure time using various cryoprotectants.

Author(s) : TAKAGI M., BOEDIONO A., SAHA S., SUZUKI T.

Type of article: Article

Summary

The relationship between cryoprotectant and exposure time on the development of IVF-derived embryos in culture after freezing and thawing was examined. Good to excellent quality day 7 to 8 blastocysts and expanded blastocysts were suspended in 1.6 M propylene glycol, 1.8 M ethylene glycol, 1.1 M diethylene glycol, and 1.3 M ethylene glycol monomethyl ether. The authors' findings suggest that the toxicity of different cryoprotectants is related to the exposure time and that propylene glycol and ethylene glycol were relatively nontoxic as is exposure to ethylene glycol monomethyl ether for short periods.

Details

  • Original title: Survival rate of frozen-thawed bovine IVF embryos in relation to exposure time using various cryoprotectants.
  • Record ID : 1994-1874
  • Languages: English
  • Source: Cryobiology - vol. 30 - n. 3
  • Publication date: 1993/06

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