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EFFECT OF FREEZE-DRYING ON PLASMIDS IN KLEBSIELLA SPECIES BY NUTRIENT GELATIN METHOD.

Author(s) : HO B.

Summary

SIXTY-EIGHT STRAINS OF KLEBSIELLA CULTURES HARBOURING MULTIPLE ANTIBIOTIC RESISTANCE PLASMIDS WERE PRESERVED BY FREEZE-DRYING USING THE NUTRIENT GELATIN DISC METHOD. THE SUSPENDING MEDIUM COMPRISED GELATIN AND INOSITOL. THE STABILITY OF THE PLASMIDS WERE EXAMINED 1 YEAR AFTER THE CULTURES WERE FREEZE-DRIED. THE MOLECULAR WEIGHTS OF THE PLASMID RANGED FROM 2 TO 150 MEGADALTONS. CULTURES BEFORE AND AFTER FREEZE-DRYING SHOWED THAT THE MOLECULAR WEIGHTS OF THE PLASMIDS WERE MAINTAINED AND THAT THERE WAS NO CHANGE IN THEIR ANTIBIOTIC SENSITIVITY PATTERNS. FREEZE-DRYING BY NUTRIENT GELATIN METHOD SHOWED PROMISING RESULTS IN MAINTENANCE OF BACTERIAL CULTURES HARBOURING MULTIPLE ANTIBIOTIC RESISTANCE PLASMIDS. FURTHERMORE THE COST IS ONE TENTH THAT OF CONVENTIONAL FREEZE-DRYING METHOD USING GLASS AMPOULES.

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Pages: 1985-1

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Details

  • Original title: EFFECT OF FREEZE-DRYING ON PLASMIDS IN KLEBSIELLA SPECIES BY NUTRIENT GELATIN METHOD.
  • Record ID : 1987-1210
  • Languages: English
  • Source: Fundamentals and Applications of Freeze-Drying to Biological Materials, Drugs and Foodstuffs.
  • Publication date: 1985/05/20
  • Document available for consultation in the library of the IIR headquarters only.

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