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FREEZE-DRY TOLERANCE OF ESCHERICHIA COLI RECA MUTANTS INFLUENCED BY SHIFT OF THE GROWTH TEMPERATURE.

Author(s) : TAKANO M., TAKEMURA H., TSUCHIDO T.

Summary

BACTERIA OF ESCHERICHIA COLI RECA STRAINS WERE VERY SUSCEPTIBLE TO FREEZE-DRYING. A SHIFT-UP OF GROWTH TEMPERATURE FROM 303 TO 315 K (30 TO 42 DEG C) IN THE LOG PHASE CULTURE WAS FOUND TO INCREASE VIABILITY AFTER FREEZE-DRYING. BACTERIA GROWN AT THE HIGHER TEMPERATURE WERE THE MORE TOLERANT TO FREEZE-DRYING. THE INCREASE OF DRY-TOLERANCE WAS OBSERVED ALSO BY TREATMENT WITH 0.1% HYDROGENPEROXIDE AND THE FOLLOWING INCUBATION AT 303 K IN A GROWTH MEDIUM FOR 40 MIN. THE BREAKAGES IN SINGLE STRAND DNA FROM THE TEMPERATURE-SHIFTED AND DRIED BACTERIA WERE SIMILAR TO THOSE FROM THE UNSHIFTED BACTERIA.

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Details

  • Original title: FREEZE-DRY TOLERANCE OF ESCHERICHIA COLI RECA MUTANTS INFLUENCED BY SHIFT OF THE GROWTH TEMPERATURE.
  • Record ID : 1987-1207
  • Languages: English
  • Source: Fundamentals and Applications of Freeze-Drying to Biological Materials, Drugs and Foodstuffs.
  • Publication date: 1985/05/20
  • Document available for consultation in the library of the IIR headquarters only.

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