Share

Effect of pretreatment conditions on the cryopreservation of in vitro-cultured yam (Dioscorea alata 'Brazo Fuerte' and D. bulbifera 'Nouméa Imboro') shoot apices by encapsulation-dehydration.

Author(s) : MALAURIE B., TROUSLOT M. F., ENGELMANN F., CHABRILLANGE N.

Type of article: Article

Summary

For D. alata, the highest survival rate (up to 67%) was obtained after a 3 to 10-day pretreatment with 0.9 and 1 mole sucrose followed by 11 to 16 hours of silica gel-dehydration, rapid cooling in liquid nitrogen and, after rewarming, subculture for 35 days on medium supplemented with growth regulators. For D. bulbifera, optimal survival (up to 65%) was achieved following pretreatment with sucrose concentrations higher than 0.75 mole, regardless of the pretreatment duration, and 14 to 16 hours of dehydration.

Details

  • Original title: Effect of pretreatment conditions on the cryopreservation of in vitro-cultured yam (Dioscorea alata 'Brazo Fuerte' and D. bulbifera 'Nouméa Imboro') shoot apices by encapsulation-dehydration.
  • Record ID : 1999-1237
  • Languages: English
  • Source: Cryo-Letters - vol. 19 - n. 1
  • Publication date: 1998/01
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (5)
See the source

Indexing