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EFFECT OF SUCROSE AND DMSO CONCENTRATIONS ON THE RESISTANCE TO FREEZING IN LIQUID NITROGEN OF TERMINAL CARNATION (DIANTHUS CARYOPHYLLUS L, VAR EOLO)MERISTEMS CULTIVATED IN VITRO.

EFFETS DES TENEURS EN SACCHAROSE ET EN DMSO SUR LA RESISTANCE A UNE CONGELATION DANS L'AZOTE LIQUIDE DE MERISTEMES TERMINAUX D'OEILLETS (DIANTHUS CARYOPHYLLUS L, VAR EOLO) CULTIVES IN VITRO.

Author(s) : DEREUDDRE J.

Summary

THE PRETREATMENT CONDITIONS RELATIVE TO CRYOPROTECTANT CONCENTRATIONS (SUCROSE AND DMSO) WERE STUDIED, IN ORDER TO DEFINE A CRYOPRESERVATION PROCESS FOR IN VITRO CULTURED CARNATION MERISTEMS. MERISTEM RESISTANCE TO DEEP FREEZING IS CLOSELY DEPENDING ON SUCROSE AND, TO A SMALLER EXTENT, ON DMSO CONCENTRATIONS. SOME OPTIMAL CONDITIONS, ENSURING A REGROWTH RATE OF 98.9%, HAVE BEEN DEFINED: PRECULTURE OF EXCISED MERISTEMS, DURING 24 HOURS, ON A MEDIUM SUPPLEMENTED WITH 0.75 M SUCROSE, FOLLOWED BY THE APPLICATION OF A SOLUTION CONTAINING 5% DMSO (V/V). A SLOW FREEZING IS USED: 0.5 K/MIN, DOWN TO 233 K (-40 DEG C), PRIOR TO IMMERSION IN LIQUID NITROGEN, AND THE SAMPLES ARE RAPIDLY REWARMED IN A 313 K (40 DEG C) WATER BATH.

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Details

  • Original title: EFFETS DES TENEURS EN SACCHAROSE ET EN DMSO SUR LA RESISTANCE A UNE CONGELATION DANS L'AZOTE LIQUIDE DE MERISTEMES TERMINAUX D'OEILLETS (DIANTHUS CARYOPHYLLUS L, VAR EOLO) CULTIVES IN VITRO.
  • Record ID : 1988-1019
  • Languages: French
  • Source: Development in refrigeration, refrigeration for development. Proceedings of the XVIIth international Congress of Refrigeration.
  • Publication date: 1987/08/24
  • Document available for consultation in the library of the IIR headquarters only.

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