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EXPERIMENTAL CRYOPRESERVATION OF THE GAMETOPHYTES OF ALGA UNDARIA PINNATIFIDA (LAMINARIALE).

ESSAI DE CRYOCONSERVATION DES GAMETOPHYTES DE L'ALGUE ALIMENTAIRE UNDARIA PINNATIFIDA (LAMINARIALE).

Author(s) : ARBAULT S.

Type of article: Article

Summary

LOW TEMPERATURE TOLERANCE OF GAMETOPHYTES OF ALGA UNDARIA PINNATIFIDA IS STUDIED UNDER DIFFERENT CRYOPRESERVATION METHODS. STORAGE AT 193 K (-80 DEG C) IN A SOLUTION CONTAINING 28% GLYCEROL, AND AT 77 K (-196 DEG C) IN A SEA WATER SOLUTION CONTAINING 5 OR 10% GLYCEROL, GIVES A SURVIVAL TIME OF 9 AND 4 DAYS. VIABILITY IS NOT AFFECTED BY A COOLING RATE OF 5 K/MIN AND BY 15 MINUTES STORAGE AT 258 K (-15 DEG C) WITHOUT A CRYOPROTECTIVE AGENT, BUT IS SIGNIFICANTLY REDUCED BY 15 MINUTES STORAGE AT 243 K (-30 DEG C) OR AT 77 K, EVEN WITH DMSO.

Details

  • Original title: ESSAI DE CRYOCONSERVATION DES GAMETOPHYTES DE L'ALGUE ALIMENTAIRE UNDARIA PINNATIFIDA (LAMINARIALE).
  • Record ID : 1991-1842
  • Languages: French
  • Source: Aquat. living Resour./Ressour. vivantes aquat. - vol. 3 - n. 3
  • Publication date: 1990
  • Document available for consultation in the library of the IIR headquarters only.

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