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REGENERATION OF PLANTS FROM FROZEN: 77 K (-196 DEG C) PROTOPLASTS OF ATROPA BELLADONNA L, DATURA INNOXIA MILL AND NICOTIANA TABACUM L.

Author(s) : BAJAJ Y. P. S.

Type of article: Article

Summary

PROTOPLASTS OBTAINED FROM MESOPHYLL CELLS AND CALLUS CELL SUSPENSIONS OF 3 ALKALOID PRODUCING PLANTS, A. BELLADONNA, D. INNOXIA AND N. TABACUM SUBJECTED TO FREEZING IN LIQUID NITROGEN (77 K) IN PRESENCE OF DMSO, MANNITOL AND SUCROSE, AND THAWED AT 308 K (35 DEG C) FORMED CALLUS AND EVENTUALLY REGENERATED SHOOTS/PLANTS. THE PLANTS FORMED NORMAL FLOWERS AND SET SEEDS. THE DEGREE OF REVIVAL AND REGENERATION VARIED A GREAT DEAL. ATROPA PROTOPLASTS GAVE THE BEST RESPONSE, FOLLOWED BY NICOTIANA AND DATURA.

Details

  • Original title: REGENERATION OF PLANTS FROM FROZEN: 77 K (-196 DEG C) PROTOPLASTS OF ATROPA BELLADONNA L, DATURA INNOXIA MILL AND NICOTIANA TABACUM L.
  • Record ID : 1989-1189
  • Languages: English
  • Source: Indian J. exp. Biol. - vol. 26 - n. 4
  • Publication date: 1988
  • Document available for consultation in the library of the IIR headquarters only.

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