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FREEZING OF ISOLATED THYLAKOID MEMBRANES IN COMPLEX MEDIA. II. SIMULATION OF THE CONDITIONS IN THE CHLOROPLAST STROMA.

Author(s) : SANTARIUS K. A.

Type of article: Article

Summary

ISOLATED SPINACH THYLAKOIDS WERE FROZEN IN AN ARTIFICIAL STROMA MEDIUM CONTAINING SUGARS, AMINO ACIDS AND INORGANIC ELECTROLYTES WHICH CORRESPONDED TO CONDITIONS EXISTING IN CHLOROPLASTS OF FROST-SENSITIVE LEAF MATERIAL. THE ANALYSIS OF THE RELATIVE CONTRIBUTION OF DIFFERENT SOLUTES TO MEMBRANE SURVIVAL REVEALED THAT CRYOPRESERVATION WAS DUE TO THE AVAILABILITY OF CRYOPROTECTANTS AND TO THE COMBINATION OF THE ESSENTIAL INORGANIC ELECTROLYTES: A SUITABLE BALANCE BETWEEN THESE POTENTIALLY CRYOTOXIC SOLUTES DIMINISHED THYLAKOID INACTIVATION. IN ADDITION, THE PRESENCE OF PROTEIN ENHANCED THYLAKOID STABILIZATION. IN INTACT LEAVES DAMAGE AND PROTECTION OF THYLAKOID MEMBRANES DURING FREEZING IS THE RESULT OF COMPLEX INTERACTIONS BETWEEN VARIOUS INDIVIDUAL FACTORS.

Details

  • Original title: FREEZING OF ISOLATED THYLAKOID MEMBRANES IN COMPLEX MEDIA. II. SIMULATION OF THE CONDITIONS IN THE CHLOROPLAST STROMA.
  • Record ID : 1986-2105
  • Languages: English
  • Source: Cryo-Letters - vol. 7 - n. 1
  • Publication date: 1986
  • Document available for consultation in the library of the IIR headquarters only.

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