Summary
The authors developed a new cryopreservation apparatus for the long-term preservation of biomaterials which incorporates auto-cascade refrigeration, automatic control and the "liquidus-tracking" method. The experiments for cooling and warming with variable concentration were conducted under the control of programs with three common cryoprotectants separately including glycerol, propylene glycol and dimethyl sulfoxide. The final solution concentration arrived at the critical concentration for vitrification and the lowest solution temperature reached -80°C. The experimental results show that the performance of the new apparatus could match the requirement of the preservation and it may have a good prospect in the vitrification preservation of biomaterials.
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Details
- Original title: [In Chinese. / En chinois.]
- Record ID : 2010-1308
- Languages: Chinese
- Source: Journal of Refrigeration - vol. 31 - n. 2
- Publication date: 2010/04
- DOI: http://dx.doi.org/10.3969/j.issn.0253-4339.2010.02.043
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Indexing
- Themes: Transport and handling of cryogenic fluids
- Keywords: Cryostat; Design; Performance; Expérimentation; Cryopreservation
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ULTRAHIGH-VACUUM CRYOSTAT AND SAMPLE MANIPULATO...
- Author(s) : SHAYEGAN M.
- Date : 1985
- Languages : English
- Source: Rev. sci. Instrum. - vol. 56 - n. 9
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The trade off of the demands and the thermal in...
- Author(s) : KAMIOKA Y.
- Date : 1993
- Languages : Japanese
- Source: Cryogenics/ Cryog. Eng. - vol. 28 - n. 8
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A SMALL CRYOSTAT FOR SUBCOOLED SUPERFLUID HELIU...
- Author(s) : WANG R.
- Date : 1990/09
- Languages : English
- Source: Cryogenics - vol. 30
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A HELIUM 3 CRYOSTAT FOR INVESTIGATION OF THERMA...
- Author(s) : JEZOWSKI A., GORODILOW B. J.
- Date : 1990
- Languages : Polish
- Source: Chlodnictwo - vol. 25 - n. 3-4
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Vacuum insulation for a cryostat.
- Author(s) : NOGUCHI T.
- Date : 1993
- Languages : Japanese
- Source: Cryogenics/ Cryog. Eng. - vol. 28 - n. 7
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