Share

Extending the viability of sea urchin gametes.

Author(s) : SPIEGLER M. A., OPPENHEIMER S. B.

Type of article: Article

Summary

The sea urchin is the material of choice for studying many early developmental events. But the eggs are easily damaged by freezing. The study was designed to extend the viability of Lytechinus pictus eggs and sperm without freezing. Gametes can be stored longer at lower temperatures than previously described. Sperm was kept viable consistently for at least 12 days with little decrease in viability when stored in glass test tubes or plastic petri dishes and submerged in ice inside a refrigerator at 0 more or less 1 deg C. Eggs were maintained from 1 to 7 days, rather than the 1 day or so previously reported, when stored in glass test tubes submerged in ice in a refrigerator at 0 more or less 1 deg C. Results of egg and sperm experiments varied at different times in the season.

Details

  • Original title: Extending the viability of sea urchin gametes.
  • Record ID : 1996-0536
  • Languages: English
  • Source: Cryobiology - vol. 32 - n. 2
  • Publication date: 1995/04

Links


See other articles in this issue (7)
See the source

Indexing