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FREEZING OF SEA URCHIN SPERM TO 77 K (-196 DEG C).

[In Russian. / En russe.]

Author(s) : MEZEVIKINA L. M., KRASTS I. V., VERPRINCEV B. N.

Type of article: Article

Summary

SPERM OF SEA URCHINS STRONGYLOCENTROTUS NUDUS AND STRONGYLOCENTROTUS INTERMEDIUS) WAS COOLED TO 77 K IN SEA WATER WITH CRYOPROTECTIVE ADDITIVES (1 M GLYCEROL, 1.5 M DMSO AND 1.5 M ETHYLENE GLYCOL). ICE CRYSTALLIZATION WAS ARTIFICIALLY INITIATED AT 266 K (-7 DEG C). UP TO 233 K (-40 DEG C), THE SPERM WAS COOLED AT A GRADUALLY INCREASING RATE FROM 0.3 TO 1.5 K/MIN. THEN THE SAMPLES WERE TRANSFERRED INTO LIQUID NITROGEN. THAWING WAS PERFORMED IN WATER BATH AT 293 K (20 DEG C). ETHYLENE GLYCOL AND DMSO PROVED TO BE THE BEST CRYOPROTECTANTS.

Details

  • Original title: [In Russian. / En russe.]
  • Record ID : 1986-2547
  • Languages: Russian
  • Source: Kriobiologija - n. 1
  • Publication date: 1986
  • Document available for consultation in the library of the IIR headquarters only.

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