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Genetic integrity of cryopreserved plant cells: a review.

Author(s) : HARDING K.

Type of article: Article

Summary

Cryopreservation techniques exists for some 100 plant species. Cryopreservation for the long-term conservation of in vitro germplasm results in the exposure of tissues to physical, chemical and physiological stresses causing cryoinjury. Although the effects of cryoinjury upon the genome are often unknown, any accumulative DNA polymorphism may not be induced by cryopreservation per se but are the result of the whole culture-cryoprotection-regeneration process. It is desirable to assess the genetic integrity of plants surviving cryogenic storage to determine if they are 'true to type' after cryopreservation. This can be done at phenotypic, histological, cytological, biochemical and molecular levels. The relevance of these approaches to stability investigations is discussed. This review provides an insight into the genetic stability and the re-introduction of cryopreserved plants into the environment.

Details

  • Original title: Genetic integrity of cryopreserved plant cells: a review.
  • Record ID : 2004-2095
  • Languages: English
  • Source: Cryo-Letters - vol. 25 - n. 1
  • Publication date: 2004/01
  • Document available for consultation in the library of the IIR headquarters only.

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