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Human corneal studies with a vitrification solution containing dimethyl sulfoxide, formamide, and 1,2-propanediol.

Author(s) : BOURNE W. M., NELSON L. R.

Type of article: Article

Summary

Immersion of the human corneas for 25 min in each of the four graded solutions at 0 deg C was required for sufficient penetration of the cryoprotectant to allow total corneal vitrification and rewarming without ice formation. The prolonged exposure caused unacceptable damage to the corneal endothelium, however. Successful vitrification of human corneas with this solution will require a safe method for obtaining corneal equilibration with the cryoprotectant.

Details

  • Original title: Human corneal studies with a vitrification solution containing dimethyl sulfoxide, formamide, and 1,2-propanediol.
  • Record ID : 1995-3877
  • Languages: English
  • Source: Cryobiology - vol. 31 - n. 6
  • Publication date: 1994/12

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