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Comparison of three methods for human corneal cryopreservation that utilize DMSO.

Author(s) : BOURNE W. M., NELSON L. R., HODGE D. O.

Type of article: Article

Summary

The authors compared endothelial cell survival in human corneas after cryopreservation by three methods that utilize DMSO. Twenty-eight human cadaver corneas were cryopreserved by one of three methods, stored briefly over liquid nitrogen, thawed, cultured at 37 °C for three days, and fixed for scanning electron microscopy. Seventeen control corneas underwent identical cryoprotectant immersion and culture protocols but were not frozen. Endothelial photographs taken after 1 and 3 days of culture were analyzed. Endothelial cell losses in cryopreserved corneas by methods 1, 2, and 3, respectively, were 36, 22 and 10% after 1 day of culture and 57, 36, and 27% after 3 days of culture. Cryopreservation by method 3 had less cell loss than methods 1 or 2 (P less than 0.02) but greater cell loss than the control corneas for method 3 (P less than 0.001). No loss of cells occurred in the control corneas for methods 1 and 3 but substantial cell loss (26%) occurred in the control corneas for method 2. The decrease in cell number that occurs in culture may represent latent cryodamage and must be understood and overcome in vivo before the technique can be used clinically.

Details

  • Original title: Comparison of three methods for human corneal cryopreservation that utilize DMSO.
  • Record ID : 2000-2242
  • Languages: English
  • Source: Cryobiology - vol. 39 - n. 1
  • Publication date: 1999/08

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