A convenient, non-invasive method for measuring the kinetics of permeation of dimethyl sulphoxide into isolated corneas using nuclear magnetic resonance spectroscopy.

Type of article: Article

Summary

Data for the kinetics of influx of cryoprotectants into tissues is essential for ensuring equilibration and for designing optimized procedures for cryopreservation. Proton nuclear magnetic resonance spectroscopy is used as a non invasive method for assessing the permeation of dimethyl suphoxide into isolated rabbit corneas at 0 deg C. Corneas reached 64% equilibration within 10 minutes and 82% after 2 hours. Permeation was significantly faster in de-epithelialized corneas.

Details

Original title: A convenient, non-invasive method for measuring the kinetics of permeation of dimethyl sulphoxide into isolated corneas using nuclear magnetic resonance spectroscopy.
Record ID : 1993-0544
Languages: English
Source: Cryo-Letters - vol. 13 - n. 4
Publication date: 1992/07
Document available for consultation in the library of the IIR headquarters only.

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Indexing

Themes: The influence of refrigeration on cells, tissues and organs
Keywords: Cryoprotectant; Cell; Measurement; DMSO; NMR; Permeability; Eye; Cornea