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IMMUNOCOMPETENCE OF THE RESIDUAL LYMPHOCYTES IN FROZEN BLOOD.

Author(s) : HARADA M.

Type of article: Article

Summary

LYMPHOCYTES REMAINING IN WASHED, PREVIOUSLY FROZEN BLOOD WERE ISOLATED AND THEIR IMMUNOCOMPETENCE WAS STUDIED ON THE BASIC OF T-AND B-CELL MARKERS, MITOGENIC RESPONSES, AND MIXED LYMPHOCYTE CULTURE (MLC). THE RESULTS SHOWED THAT FREEZE-PRESERVATION OF RED CELLS CONSIDERABLY DETERIORATED IMMUNOCOMPETENCE OF THE RESIDUAL LYMPHOCYTES IN ADDITION TO PREFERENTIAL REMOVAL OF POLYMORPHONUCLEAR CELLS. THESE LYMPHOCYTES SHOWED WEAK PROLIFERATION TO PHYTOHEMAGGLUTININ AND CONCANAVALIN A STIMULATION, BUT FAILED TO RESPOND TO ALLOANTIGENS IN MLC. THEY RETAINED STIMULATING ACTIVITY TO ALLOGENEIC LYMPHOCYTES IN MLC. THESE OBSERVATIONS SUGGEST THAT SOME PORTION OF THE RESIDUAL LYMPHOCYTES MAY REMAIN VIABLE ENOUGH TO POSSESS IMMUNOCOMPETENCE AND RECOGNIZABLE IMMUNOGENICITY.

Details

  • Original title: IMMUNOCOMPETENCE OF THE RESIDUAL LYMPHOCYTES IN FROZEN BLOOD.
  • Record ID : 1981-0302
  • Languages: English
  • Source: Cryobiology - vol. 17 - n. 2
  • Publication date: 1980/04

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