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In vitro and in vivo survival of cryopreserved sheep embryos.

Author(s) : SONGSASEN N., BUCKRELL B. C., PLANTE C., LEIBO S. P.

Type of article: Article

Summary

The respective survivals of embryos frozen in ethylene glycol, propylene glycol, and DMSO were 76.9, 62.5, and 55.6%, based on their development into hatched blastocysts in vitro. 67 embryos frozen in ethylene glycol were thawed and directly diluted with phosphate-buffered saline; 47 of these (70%) appeared morphologically normal and were transferred into 14 recipients. Five of these recipients, which had received a total of 16 embryos, became pregnant. Ten lambs were born. Using ethylene glycol has potential application for practical field use.

Details

  • Original title: In vitro and in vivo survival of cryopreserved sheep embryos.
  • Record ID : 1995-3879
  • Languages: English
  • Source: Cryobiology - vol. 32 - n. 1
  • Publication date: 1995/02

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