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VITRIFICATION OF HUMAN MONOCYTES.

Author(s) : TAKAHASHI T.

Type of article: Article

Summary

HUMAN MONOCYTES PURIFIED FROM PERIPHERAL BLOOD BY COUNTERFLOW CENTRIFUGAL ELUTRIATION WERE CRYOPRESERVED IN A VITREOUS STATE AT 101 KILOPASCALS PRESSURE. THE VITRIFICATION SOLUTION WAS HANKS' BALANCED SALT SOLUTION CONTAINING (W/V) 20.5% DMSO, 15.5% ACETAMIDE, 10% PROPYLENE GLYCOL, AND 6% POLYETHYLENE GLYCOL. THE NUMERICAL CELL RECOVERY WAS ABOUT 92% AND MOST OF THESE RETAINED NORMAL PHAGOCYTIC AND CHEMOTACTIC ABILITY. DIFFERENTIAL SCANNING CALORIMETER RECORDS OF THE SOLUTION SHOW A GLASS TRANSITION AT 158 K (-115 DEG C) DURING COOLING AND WARMING, BUT NO EVIDENCE OF ICE FORMATION DURING COOLING. DEVITRIFICATION OCCURS AT ABOUT 203 K (-70 DEG C) DURING WARMING AT RATES AS RAPID AS 80 K/MIN.

Details

  • Original title: VITRIFICATION OF HUMAN MONOCYTES.
  • Record ID : 1986-2542
  • Languages: English
  • Source: Cryobiology - vol. 23 - n. 2
  • Publication date: 1986

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