ShareVITRIFICATION OF HUMAN MONOCYTES.
Author(s) : TAKAHASHI T.
Type of article: Article
Summary
HUMAN MONOCYTES PURIFIED FROM PERIPHERAL BLOOD BY COUNTERFLOW CENTRIFUGAL ELUTRIATION WERE CRYOPRESERVED IN A VITREOUS STATE AT 101 KILOPASCALS PRESSURE. THE VITRIFICATION SOLUTION WAS HANKS' BALANCED SALT SOLUTION CONTAINING (W/V) 20.5% DMSO, 15.5% ACETAMIDE, 10% PROPYLENE GLYCOL, AND 6% POLYETHYLENE GLYCOL. THE NUMERICAL CELL RECOVERY WAS ABOUT 92% AND MOST OF THESE RETAINED NORMAL PHAGOCYTIC AND CHEMOTACTIC ABILITY. DIFFERENTIAL SCANNING CALORIMETER RECORDS OF THE SOLUTION SHOW A GLASS TRANSITION AT 158 K (-115 DEG C) DURING COOLING AND WARMING, BUT NO EVIDENCE OF ICE FORMATION DURING COOLING. DEVITRIFICATION OCCURS AT ABOUT 203 K (-70 DEG C) DURING WARMING AT RATES AS RAPID AS 80 K/MIN.
Details
- Original title: VITRIFICATION OF HUMAN MONOCYTES.
- Record ID : 1986-2542
- Languages: English
- Source: Cryobiology - vol. 23 - n. 2
- Publication date: 1986
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