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EFFECTS OF VARIOUS CRYOPROTECTIVE AGENTS ON THE SURVIVAL OF UNFROZEN AND FROZEN MOUSE EMBRYOS.

Author(s) : KASAI M., NIWA K., IRITANI A.

Type of article: Article

Summary

ALL AGENTS HAVE A DELETERIOUS EFFECT ON THE SURVIVAL OF UNFROZEN MORULA AT 293K (20 DEGC). THE NOXIOUS EFFECT OF ERYTHRITOL IS LOWER AT 273K (0 DEGC) AND SUCROSE HAS A PROTECTIVE EFFECT AT THIS TEMPERATURE. DIMETHYLFORMAMIDE AND DMSO ARE MORE NOXIOUS THAN ETHYLENE GLYCOL AND GLYCEROL AT BOTH TEMPERATURES. SLOW FREEZING IN PRESENCE OF BOTH LATTER GIVES 80 TO 94% SURVIVAL. SUCROSE AND DIMETHYLFORMAMIDE HAVE NO CRYOPROTECTIVE EFFECT. (Bull. CNRS, FR., 81-361-17971.

Details

  • Original title: EFFECTS OF VARIOUS CRYOPROTECTIVE AGENTS ON THE SURVIVAL OF UNFROZEN AND FROZEN MOUSE EMBRYOS.
  • Record ID : 1982-1767
  • Languages: English
  • Source: J. Reprod. Fertil. - vol. 63 - n. 1
  • Publication date: 1981

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