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Cryopreservation of a marine microalga, Nannochloropsis oculata (Eustigmatophyceae).

Author(s) : GWO J. C., CHIU J. Y., CHOU C. C., et al.

Type of article: Article

Summary

The cryopreservation of algae could prevent genetic drift and minimize labour costs compared with the current method of maintenance and subculturing. Clear, simple protocols for cryopreservation of marine microalga, Nannochloropsis oculata were developed and cryoprotectant choice and concentration optimised. The viability of the microalga was assessed directly after thawing, and algal concentration was measured after 2-30 days of growth. Five cryoprotectants (DMSO, ethylene glycol, glycerol, methanol, and propylene glycol) at five concentrations (10, 20, 30, 40, and 50%; v/v) were evaluated to determine the toxicity of various cryoprotectants to N. oculata. Direct freezing of algae in liquid nitrogen resulted in a severe loss of viability and a modified cryopreservation protocol proved to be more appropriate for the preservation of N. oculata. Cryopreservation protocols developed and tested in the study might be applied to cryopreserving other strains, or species, in this genus.

Details

  • Original title: Cryopreservation of a marine microalga, Nannochloropsis oculata (Eustigmatophyceae).
  • Record ID : 2005-2846
  • Languages: English
  • Source: Cryobiology - vol. 50 - n. 3
  • Publication date: 2005/06

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