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Instant cryopreservation of porcine heart valves and aortic segments in low-viscous polydimethylsiloxane - first results.

Number: pap. n. 0572

Author(s) : LAUK-DUBITSKIY S., SERVERGINA L., KOROVIN I., et al.

Summary

The aim of study was to evaluate the safety of heart valves cryopreservation in low-viscous polydimethylsiloxane (PDMS). 15 porcine heart valves (mitral and aortic / 1.0 cm) and aortic segments (2.0 cm) were cryopreserved in PDMS (1cSt, -75°C, 1L, temperature control) with stirring (150 turns per minute) for 40 sec, and then thawed in the PDMS (5cSt, +24°C, 1L, 1 min). The cryopreservation protocol was applied with speed of freezing and thawing close to 200°C/min without cryoprotectants and LN2. The quality of cryopreservation was assessed by histological methods (Orcein, Van Gieson, H&E). There were no critical differences between the valves and aortic segments of experimental and control groups. Using of PDMS is feasible for instant cryopreservation of vascular tissue for their implantation as homografts or biobanking with possible additional usage of the inner hydrophilic covering of tissue to prevent hydrophobization.

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Details

  • Original title: Instant cryopreservation of porcine heart valves and aortic segments in low-viscous polydimethylsiloxane - first results.
  • Record ID : 30025894
  • Languages: English
  • Source: Proceedings of the 25th IIR International Congress of Refrigeration: Montréal , Canada, August 24-30, 2019.
  • Publication date: 2019/08/24
  • DOI: http://dx.doi.org/10.18462/iir.icr.2019.0572

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