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Integrity of endothelium in cryopreservation human cornea.

Author(s) : NERONOV A., MAZGALOVA J., CHOLAKOVA M., et al.

Type of article: Article

Summary

The aim of the present study was to elaborate an optimal method for cryopreservation of human donor cornea for transplantation and to follow the morphological changes in the structure of the endothelial cell layer using scanning electron microscopy. Sixteen groups, with four donor cornea each, were cryopreserved at cooling rates of 1 and 5 °C/min. Four cryoprotectants (glycerol, DMSO, 1,2-propanediol, polyethylene glycol-400) in two concentrations, 5 and 10% v/v, were prepared on the bases of medium Optisol GS supplied with 20% v/v human serum albumin. Four human cornea were used as controls. Endothelial cell recovery of the cornea after thawing and 24 hours culture was calculated as a percent of the preserved recovered cells. Sufficient recovery of the endothelial cell layer, making the cornea suitable for transplantation was obtained using the cryoprotectants DMSO and especially polyethylene glycol-400.

Details

  • Original title: Integrity of endothelium in cryopreservation human cornea.
  • Record ID : 2006-0467
  • Languages: English
  • Source: CryoLetters - vol. 26 - n. 2
  • Publication date: 2005/03
  • Document available for consultation in the library of the IIR headquarters only.

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