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MANIFESTATIONS OF CELL DAMAGE AFTER FREEZING AND THAWING.

Author(s) : MCGANN L. E., YANG H., WALTERSON M.

Type of article: Article

Summary

HAMSTER FIBROBLASTS, HUMAN LYMPHOCYTES AND GRANULOCYTES WERE SUBJECTED TO A GRADED FREEZE-THAW STRESS IN THE ABSENCE OF CRYOPROTECTIVE COMPOUND BY COOLING AT 1 K/MIN TO BETWEEN 263 AND 233 K (-10 AND -40 DEG C), AND THEN WERE EITHER WARMED DIRECTLY IN WATER AT 310 K (37 DEG C) OR COOLED RAPIDLY TO 77 K (-196 DEG C) BEFORE RAPID WARMING. MITOCHONDRIAL FUNCTION IN THE CELLS WAS THEN ASSESSED. CELLS BEHAVED AS OSMOMETERS AFTER COOLING AT 1 K/MIN TO LOW TEMPERATURES, INDICATING THAT THE PLASMA MEMBRANE IS NOT A PRIMARY SITE FOR INJURY SUSTAINED DURING SLOW COOLING. FEWER CELLS COULD RESPOND OSMOTICALLY AFTER COOLING UNDER CONDITIONS WHERE INTRACELLULAR FREEZING WAS LIKELY, IMPLYING THAT THE PLASMA MEMBRANE IS DIRECTLY DAMAGED BY THE CONDITIONS LEADING TO INTRACELLULAR FREEZING. A GENERAL MODEL OF FREEZING INJURY TO NUCLEATED MAMMALIAN CELLS IS PROPOSED IN WHICH DISRUPTION OF THE LYSOSOMES CONSTITUTES THE PRIMARY LESION.

Details

  • Original title: MANIFESTATIONS OF CELL DAMAGE AFTER FREEZING AND THAWING.
  • Record ID : 1989-0788
  • Languages: English
  • Source: Cryobiology - vol. 25 - n. 3
  • Publication date: 1988

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