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MAXIMUM SURVIVAL OF FROZEN GOAT EMBRYOS IS ATTAINED AT THE EXPANDED, HATCHING AND HATCHED BLASTOCYST STAGES OF DEVELOPMENT.

Author(s) : LI R.

Type of article: Article

Summary

EMBRYOS WERE COLLECTED 5, 6 AND 7 DAYS AFTER OVULATION TO DETERMINE THEIR OPTIMUM STAGE OF DEVELOPMENT FOR CRYOPRESERVATION. THE EMBRYOS WEREFROZEN USING EITHER GLYCEROL OR DMSO AND THEN THAWED AND CULTURED TO DETERMINE THEIR VIABILITY. THE RATE OF SURVIVAL INCREASED AS THE STAGE OF DEVELOPMENT OF THE EMBRYOS INCREASED. ALL HATCHED BLASTOCYSTS SURVIVED FREEZING REGARDLESS OF WHICH CRYOPROTECTANT WAS USED, WHEREAS NONE OF THE 22 MORULAE FROZEN WITH DMSO SURVIVED, AND ONLY 12 OF THE 63 MORULAE FROZEN WITH GLYCEROL SURVIVED.

Details

  • Original title: MAXIMUM SURVIVAL OF FROZEN GOAT EMBRYOS IS ATTAINED AT THE EXPANDED, HATCHING AND HATCHED BLASTOCYST STAGES OF DEVELOPMENT.
  • Record ID : 1991-0890
  • Languages: English
  • Source: Reprod. Fertil. Dev. - vol. 2 - n. 4
  • Publication date: 1990
  • Document available for consultation in the library of the IIR headquarters only.

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