RAPID FREEZING OF THE MOUSE BLASTOCYST: EFFECTS OF CRYOPROTECTANTS AND OF TIME AND TEMPERATURE OF EXPOSURE TO CRYOPROTECTANT BEFORE DIRECT PLUNGING INTO LIQUID NITROGEN.

Type of article: Article

Summary

THE STUDY INVESTIGATES THE EFFECTS OF TIME AND TEMPERATURE OF EXPOSURE TO A HIGH CONCENTRATION (4.5 M) OF DMSO, GLYCEROL, 1,2-PROPANEDIOL, OR A MIXTURE OF DMSO AND GLYCEROL IN A SOLUTION CONTAINING 0.25 M SUCROSE, ONTHE SURVIVAL AND DEVELOPMENT OF RAPIDLY FROZEN MOUSE BLASTOCYSTS. EMBRYOS HAD SIGNIFICANTLY (P LOWER THAN 0.01) HIGHER RATES OF SURVIVAL AND DEVELOPMENT WHEN EXPOSED TO CRYOPROTECTANT AT 273 K (0 DEG C) COMPARED WITH ROOM TEMPERATURE. THE TIME OF EXPOSURE TO CRYOPROTECTANT AT EITHER 273 K OR ROOM TEMPERATURE BEFORE BEING PLUNGED INTO LIQUID NITROGEN SIGNIFICANTLY AFFECTED THE SURVIVAL AND DEVELOPMENT OF FROZEN-THAWED EMBRYOS.

Details

Original title: RAPID FREEZING OF THE MOUSE BLASTOCYST: EFFECTS OF CRYOPROTECTANTS AND OF TIME AND TEMPERATURE OF EXPOSURE TO CRYOPROTECTANT BEFORE DIRECT PLUNGING INTO LIQUID NITROGEN.
Record ID : 1992-0419
Languages: English
Source: Reprod. Fertil. Dev. - vol. 3 - n. 2
Publication date: 1991
Document available for consultation in the library of the IIR headquarters only.

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Indexing

Themes: The influence of refrigeration on cells, tissues and organs
Keywords: Cryoprotectant; DMSO; Survival; Mouse; Glycerol; Embryo; Cryopreservation