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COOLING OF EMBRYONIC CELLS, ISOLATED BLASTODERMS, AND INTACT EMBRYOS OF THE ZEBRA FISH BRACHYDANIO RERIO TO 77 K (-196 DEG C).

Author(s) : HARVEY B.

Type of article: Article

Summary

CELLS FROM THE DEVELOPING EMBRYO OF THE ZEBRA FISH SURVIVE FREEZING WHEN PROTECTED WITH 1 M DMSO AND COOLED TO 77 K IN TWO STEPS. SURVIVAL DROPS FROM 85 AND 26% WHEN CLUMPS OF 5-10 CELLS ARE SIMILARLY FROZEN, AND TO 2% WHEN ISOLATED BLASTODERMS ARE TREATED IN THE SAME WAY. THIS DECREASE IN SURVIVAL IS INTERPRETED AS AN EXAMPLE OF THE < SCALE-UP PROBLEM >, IN WHICH DIFFUSIONAL BARRIERS PREVENT CRYOPROTECTANT EQUILIBRATION AND OSMOTIC DEHYDRATION IN LARGE CELL ASSEMBLAGES. INTACT EMBRYOS PROTECTED WITH GLYCEROL (2.8 M) WELL TOLERATE SLOW COOLING TO 77 K. GLYCEROL COULD BE REMOVED FROM CELLS BY DISRUPTING THE EMBRYO SO THAT DIFFUSIONAL BARRIERS WERE REMOVED. DMSO (2.8 M) WAS INEFFECTIVE.

Details

  • Original title: COOLING OF EMBRYONIC CELLS, ISOLATED BLASTODERMS, AND INTACT EMBRYOS OF THE ZEBRA FISH BRACHYDANIO RERIO TO 77 K (-196 DEG C).
  • Record ID : 1984-1247
  • Languages: English
  • Source: Cryobiology - vol. 20 - n. 4
  • Publication date: 1983

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