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CRYOPRESERVATION OF MURINE EMBRYOS WITH TREHALOSE AND GLYCEROL.

Author(s) : HONADEL T. E., KILLIAN G. J.

Type of article: Article

Summary

SEVERAL CONCENTRATIONS OF TREHALOSE (0.0, 0.04, 0.1, 0.25 M) IN COMBINATION WITH 3 CONCENTRATIONS OF GLYCEROL (1.0, 1.5, 2.0 M) WERE EVALUATED. EMBRYOS WERE TRANSFERRED THROUGH INCREASING CONCENTRATIONS OF GLYCEROL IN DULBECCO'S PHOSPHATE-BUFFERED SALINE WITH 10% FETAL CALF SERUM TO REACH THE FINAL GLYCEROL CONCENTRATIONS. THEY WERE THEN RANDOMLY ASSIGNED TO ONE OF THE CONCENTRATIONS OF TREHALOSE. HIGHEST VIABILITY (70.0%) AFTER 48 HR IN CULTURE WAS OBTAINED FOR EMBRYOS FROZEN IN 1.5 M GLYCEROL PLUS 0.10 M TREHALOSE AS COMPARED TO 31% VIABILITY FOR EMBRYOS FROZEN WITH GLYCEROL ALONE.

Details

  • Original title: CRYOPRESERVATION OF MURINE EMBRYOS WITH TREHALOSE AND GLYCEROL.
  • Record ID : 1989-2082
  • Languages: English
  • Source: Cryobiology - vol. 25 - n. 4
  • Publication date: 1988

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